Sokoine University of Agriculture

Reduction in genome size and DNA methylation alters plant and fruit development in tissue culture induced off-type banana (Musa spp.)

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dc.contributor.author Msogoya, T. J.
dc.contributor.author Grout, B. W.
dc.contributor.author Roberts, A.
dc.date.accessioned 2019-07-26T15:41:33Z
dc.date.available 2019-07-26T15:41:33Z
dc.date.issued 2011
dc.identifier.issn 2071 - 7024
dc.identifier.uri http://www.suaire.suanet.ac.tz:8080/xmlui/handle/123456789/2877
dc.description Journal of Animal & Plant Sciences, 2011. Vol. 11, Issue 3: 1450-1456 en_US
dc.description.abstract Shoot tip in vitro micropropagation of banana (Musa AAA East Africa) cv.’ Uganda’ resulted in off-type plants with an altered plant and fruit development. This study was carried out to determine epigenetic mechanisms underlying the altered plant and fruit development of the in vitro derived off-type banana. The off-type banana was compared with in vitro micropropagation (MP) derived normal banana and conventionally propagated (CP) banana plants with no tissue culture history in their ancestry as controls. Plant development was estimated based on plant height, girth and number of days from planting to flowering. Fruit development was measured as the number of days from flowering to fruit maturation and senescence. Mechanisms underlying the altered plant and fruit development were determined based on global cytosine DNA methylation and 2C nuclear DNA content. Leaf cytosine DNA methylation and 2C nuclear DNA content were determined using reversed phase HPLC and flow cytometer, respectively. Results showed that the off-type banana was significantly (P < 0.05) taller and delayed to flower compared with the MP and CP derived banana. Similarly, the fruits of the off-type banana had significantly (P< 0.05) longer maturation, ripening and senescence periods than those of the true-totype fruits. The offtype derived plants had lower (P < 0.05) leaf global cytosine DNA methylation and 2C nuclear DNA amount compared with the MP and CP derived plants. These findings suggest that the altered plant and fruit development of the of-type banana are possibly under the control of reduced cytosine DNA methylation and nuclear DNA content. Further studies are required to identify specific genes which affect plant and fruit development upon undergoing demethylation. en_US
dc.language.iso en en_US
dc.publisher JAPS en_US
dc.subject Plant development en_US
dc.subject Fruit development en_US
dc.subject DNA methylation en_US
dc.subject Nuclear DNA content en_US
dc.subject In vitro induced off-type banana en_US
dc.title Reduction in genome size and DNA methylation alters plant and fruit development in tissue culture induced off-type banana (Musa spp.) en_US
dc.type Article en_US
dc.url http://www.biosciences.elewa.org/JAPS en_US


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